신소재공학과

Seminar

Date 20180220 

■ Title : Hydrogel-based tissue imaging:

            How materials science is revolutionizing neuroscience, biology, and medicine

 

■ Speaker : Prof. Jae-Byum Chang (Sungkyunkwan Univ.) 


■ Date and time : 20th of February, 2018 (Tue) 16:00 

 

■ Venue : Applied Engineering Department (W1-1) Room 2427 

 

■ Abstract :

Our understanding on human organs is very limited. Human organs are made of millions of cells and the molecular profiles of cells, such as numbers and sub-cellular locations of various biomolecules (proteins, mRNAs, DNAs, etc.) are precisely controlled over an entire organ. As a result, extracting the complete profiles of biological molecules within an entire organ is essential to understanding the molecular mechanisms of organ function. To achieve such goal, new optical imaging technique, which can image multiple molecular species with a single-molecule resolution over a large volume, is needed.

We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease. In addition, as iExM can be applied to various organs, such as heart, liver, pancreas, lung, intestine, they could be a very useful tool to study and diagnose various diseases, such as cancers and cardiovascular diseases.

No. Subject Author Date Views
Notice 6/5 (Tue) Prof. Sunghwan Lee (Baylor University) ADMINI 2018.06.05 81
Notice 5/29 (Tue) Prof. Sang Ho Oh (Yonsei Univ. College of Medicine) ADMINI 2018.05.29 89
Notice 5/15 (Tue) Prof. Myung Gil Kim (Dept. of Chemistry, Chung-Ang Univ.) ADMINI 2018.05.15 304
Notice 5/8 (Tue) Prof. Ju-Young Kim (UNIST MSE) ADMINI 2018.05.09 373
Notice 5/1 (Tue) Prof. Duk-Yong Yoon (KAIST MSE) ADMINI 2018.04.30 540
Notice 4/24 (Tue) Kyeong Ho Chang (CEO, Innox Advanced Materials) ADMINI 2018.04.23 514
Notice 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 595
Notice 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 480
Notice 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 450
Notice 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 319
Notice 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 316
Notice 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 281
Notice 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 341
» 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 309
264 2015 The Fall Semester Seminar file TeamLeader 2015.03.04 18979
263 2/25 (월) 홍승범박사 (Argonne National Laboratory) Administrator 2013.02.06 17964
262 [공지] 2011년 가을학기 신소재공학과 정기세미나 안내 file Administrator 2011.11.04 15313
261 3/20 (화) 정우철 박사 (California Institute of Technology) file Administrator 2012.03.15 15297
260 [공지] 2012년 봄학기 신소재공학과 정기세미나 안내 file Administrator 2012.02.13 14412
259 5/15 (화) 조문호 교수 (연세대학교 신소재공학과) Administrator 2012.05.09 13984
258 9/6 (화) 남윤성 교수 (KAIST 신소재공학과) file Administrator 2011.11.04 13931
257 [WCU-FS 사업단 세미나] 6/7 (화) 조정대 박사 (한국기계연구원) Administrator 2011.11.04 13536
256 10/19 (수) Dr. Miso Kim (MIT) Administrator 2011.11.04 11786
255 10/16 (화) 유현용 교수 (고려대학교 전기전자전파공학부) Administrator 2012.10.12 11753
254 3/19 (화) 신병하 박사 (IBM T.J. Watson Research Center) Administrator 2013.03.19 11480
253 10/11 (화) 조성준 교수 (전남대학교 응용화학공학부 file Administrator 2011.11.04 11363
252 10/18 (화) 이윤정 교수 (한양대학교 WCU, 에너지공학과) file Administrator 2011.11.04 11344
251 11/15 (화) 김진웅 교수 (한양대학교 응용화학과) file Administrator 2011.12.05 11256
250 11/1 (화) 윤명한 교수 (광주과학기술원 신소재공학부) file Administrator 2011.11.04 11254
249 3/5 (화) 정우철 교수 (KAIST 신소재공학과) Administrator 2013.03.05 11220
248 2/14 (화) 신종화 교수 (KAIST 신소재공학과) file Administrator 2012.02.15 11153
247 12/13 (화) Prof. Aloysius Soon (연세대학교 신소재공학과) file Administrator 2011.12.07 10712
246 [WCU-FS 사업단 세미나] 6/29 (수) 16:00 Prof. Digby D. Macdonald (Pennsylvania State University) Administrator 2011.11.04 10483
245 4/19 (화) 남기태 교수 (서울대학교 재료공학부) Administrator 2011.11.04 10406