신소재공학과

Seminar

Extra Form
Date 20171114

■ Title:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ Speaker: Prof. Jae-Byum Chang (Dept. of Biomedical Engineering, Sungkyunkwan Univ)  

 

■ Date and time: 14th of November, (Tue) 16:00

 

■ Venue: Applied Engineering Dpt. Bd W1 Multimedia Lecture Hall (1st Floor)

 

 Host : Prof. Yeon Sik Jung

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.



List of Articles
No. Category Subject Author Date Views
Notice Regular 03/26 (Tue) Prof. Jae-Byum Chang (KAIST MSE) ADMINI 2019.03.22 9
Notice Regular 03/19 (Tue) Dr. YangKoo Cho (Korea Research Institute of Standard and Science) ADMINI 2019.03.18 95
Notice Regular 3/12 (Tue) Prof. Chang Dong Yoo (KAIST EE) ADMINI 2019.03.12 93
Notice Regular 2/26 (Tue) Dr. Hye-Young Kim (Senior Venture Association) ADMINI 2019.02.22 201
Notice Special 1/28 (Mon) Prof. Yongjin Lee (Shanghai Tech Univ.) ADMINI 2019.01.23 518
Notice Regular 12/4 (Tue) Prof. Doo Seok Jeong (Hanyang Univ., Department of MSE) ADMINI 2018.11.30 864
Notice Regular 11/27 (Tue) Prof. Seong-Jun Jeong (Soongsil Univ.) ADMINI 2018.11.26 709
249 Special 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 540
248 Special 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 540
247 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 552
246 Regular 11/28 (Tue) Dr. Woo-Jin Lee (Ministry of Science and ICT.) ADMINI 2017.11.24 1203
245 Special 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 878
244 Special 11/21 (Tue) Dr. Young Ki Lee (ETRI) file ADMINI 2017.11.20 1044
243 Special 11/22 (Wed) Prof. Sung Hoon Kang (Johns Hopkins University) file ADMINI 2017.11.20 1280
242 Regular 11/21 (Tue) Dr. Byoung Koun Min, (KIST) ADMINI 2017.11.17 1456
241 Special 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 1524
240 Special 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 786
» Regular 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 755
238 Regular 11/7 (Tue) Prof. Su-Mi Hur (Chonnam National University) ADMINI 2017.11.05 603
237 Regular 10/31 (Tue) Director Jang Sae Young (Samsung Electronics) ADMINI 2017.10.26 616
236 Special 11/6 (Mon) Prof. Redouane Borsali (Grenoble Alpes University) ADMINI 2017.10.26 773
235 Special 11/6 ~ 7 Prof. Erik Luijten (Northwestern University) file ADMINI 2017.10.25 642
234 Special 10/23 ~ 24 Prof. Paul C. McIntyre (Stanford University) file ADMINI 2017.10.25 566
233 Regular 10/24 (Tue) Prof. Sun-Jae Kim (Sejong, Univ) ADMINI 2017.10.20 1006
232 Special 10/19 (Thu) Prof. Sang Cheol, Jung (Suncheon University) ADMINI 2017.10.18 1328
231 Regular 10/10 (Tue) Dr. Joo Choi (POSCO) ADMINI 2017.09.29 2253
230 Regular 9/26 (Tue) Prof. Tae-Woo Lee (Seoul National University) ADMINI 2017.09.22 1484
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