신소재공학과

Seminar

Extra Form
Date 20171114

■ Title:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ Speaker: Prof. Jae-Byum Chang (Dept. of Biomedical Engineering, Sungkyunkwan Univ)  

 

■ Date and time: 14th of November, (Tue) 16:00

 

■ Venue: Applied Engineering Dpt. Bd W1 Multimedia Lecture Hall (1st Floor)

 

 Host : Prof. Yeon Sik Jung

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.



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Notice Regular 5/17 (Tue) Prof. Su-Hyun Gong (Dept. of Physics, Korea Univ.) ADMINI 2019.05.10 167
Notice Special 5/7 (Tue) Prof. Dr. -ing. Dierk Raabe (MPIE) ADMINI 2019.04.30 111
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Notice Regular 4/23 (Tue) Prof. Seungwu Han (Dept. of MSE, SNU) ADMINI 2019.04.19 123
Notice Regular 4/9 (Tue) Prof. Ji Young Jo (GIST, School of MSE) ADMINI 2019.04.05 142
Notice Regular 4/2 (Tue) Prof. Li Oi Lun (Dept. of MSE, Pusan National Univ.) ADMINI 2019.03.29 177
256 Regular 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 657
255 Regular 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 578
254 Regular 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 542
253 Regular 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 444
252 Regular 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 518
251 Special 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 489
250 Special 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) file ADMINI 2018.02.05 521
249 Special 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 546
248 Special 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 546
247 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 559
246 Regular 11/28 (Tue) Dr. Woo-Jin Lee (Ministry of Science and ICT.) ADMINI 2017.11.24 1214
245 Special 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 890
244 Special 11/21 (Tue) Dr. Young Ki Lee (ETRI) file ADMINI 2017.11.20 1052
243 Special 11/22 (Wed) Prof. Sung Hoon Kang (Johns Hopkins University) file ADMINI 2017.11.20 1285
242 Regular 11/21 (Tue) Dr. Byoung Koun Min, (KIST) ADMINI 2017.11.17 1478
241 Special 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 1696
240 Special 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 792
» Regular 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 759
238 Regular 11/7 (Tue) Prof. Su-Mi Hur (Chonnam National University) ADMINI 2017.11.05 612
237 Regular 10/31 (Tue) Director Jang Sae Young (Samsung Electronics) ADMINI 2017.10.26 621
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