신소재공학과

Seminar

Date 20180220 

■ Title : Hydrogel-based tissue imaging:

            How materials science is revolutionizing neuroscience, biology, and medicine

 

■ Speaker : Prof. Jae-Byum Chang (Sungkyunkwan Univ.) 


■ Date and time : 20th of February, 2018 (Tue) 16:00 

 

■ Venue : Applied Engineering Department (W1-1) Room 2427 

 

■ Abstract :

Our understanding on human organs is very limited. Human organs are made of millions of cells and the molecular profiles of cells, such as numbers and sub-cellular locations of various biomolecules (proteins, mRNAs, DNAs, etc.) are precisely controlled over an entire organ. As a result, extracting the complete profiles of biological molecules within an entire organ is essential to understanding the molecular mechanisms of organ function. To achieve such goal, new optical imaging technique, which can image multiple molecular species with a single-molecule resolution over a large volume, is needed.

We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease. In addition, as iExM can be applied to various organs, such as heart, liver, pancreas, lung, intestine, they could be a very useful tool to study and diagnose various diseases, such as cancers and cardiovascular diseases.

No. Subject Author Date Views
Notice 7/13 (Fri) Prof. Dong Ryeol Whang (Johannes Kepler University Linz, Austria) ADMINI 2018.07.09 10
Notice 6/5 (Tue) Prof. Sunghwan Lee (Baylor University) ADMINI 2018.06.05 216
Notice 5/29 (Tue) Prof. Sang Ho Oh (Yonsei Univ. College of Medicine) ADMINI 2018.05.29 216
Notice 5/15 (Tue) Prof. Myung Gil Kim (Dept. of Chemistry, Chung-Ang Univ.) ADMINI 2018.05.15 435
261 5/8 (Tue) Prof. Ju-Young Kim (UNIST MSE) ADMINI 2018.05.09 501
260 5/1 (Tue) Prof. Duk-Yong Yoon (KAIST MSE) ADMINI 2018.04.30 654
259 4/24 (Tue) Kyeong Ho Chang (CEO, Innox Advanced Materials) ADMINI 2018.04.23 557
258 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 644
257 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 522
256 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 491
255 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 363
254 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 359
253 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 322
252 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 387
» 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 352
250 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) file ADMINI 2018.02.05 344
249 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 424
248 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 445
247 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 465
246 11/28 (Tue) Dr. Woo-Jin Lee (Ministry of Science and ICT.) ADMINI 2017.11.24 1141