신소재공학과

Seminar

Views 452
Extra Form
Date 20180220

■ Title : Hydrogel-based tissue imaging:

            How materials science is revolutionizing neuroscience, biology, and medicine

 

■ Speaker : Prof. Jae-Byum Chang (Sungkyunkwan Univ.) 


■ Date and time : 20th of February, 2018 (Tue) 16:00 

 

■ Venue : Applied Engineering Department (W1-1) Room 2427 

 

■ Abstract :

Our understanding on human organs is very limited. Human organs are made of millions of cells and the molecular profiles of cells, such as numbers and sub-cellular locations of various biomolecules (proteins, mRNAs, DNAs, etc.) are precisely controlled over an entire organ. As a result, extracting the complete profiles of biological molecules within an entire organ is essential to understanding the molecular mechanisms of organ function. To achieve such goal, new optical imaging technique, which can image multiple molecular species with a single-molecule resolution over a large volume, is needed.

We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease. In addition, as iExM can be applied to various organs, such as heart, liver, pancreas, lung, intestine, they could be a very useful tool to study and diagnose various diseases, such as cancers and cardiovascular diseases.


List of Articles
No. Category Subject Author Date Views
Notice Regular 12/4 (Tue) Prof. Doo Seok Jeong (Hanyang Univ., Department of MSE) ADMINI 2018.11.30 106
Notice Regular 11/27 (Tue) Prof. Seong-Jun Jeong (Soongsil Univ.) ADMINI 2018.11.26 108
Notice Regular 11/20 (Tue) Prof. Youn Soo Kim (Department of MSE, POSTECH) ADMINI 2018.11.19 164
Notice Special 11/14 (Wed) Prof. Hui-Ming Cheng (Director and Professor, Tsinghua University, IMR CAS) ADMINI 2018.11.13 140
Notice Special 11/20 (Tue) Prof. Alfonso H. W. Ngan (Department of Mechanical Engineering, University of Hong Kong) file ADMINI 2018.11.13 151
Notice Regular 11/13 (Tue) Dr. Kang-Wook Lee (SK Hynix) ADMINI 2018.11.13 104
Notice Regular 11/6 (Tue) Prof. Dongchan Jang (KAIST NQE) ADMINI 2018.11.02 177
Notice Special 11/2 (Fri) Dr. Sungbong Park (Intel Corporation) ADMINI 2018.10.30 206
264 Regular 6/5 (Tue) Prof. Sunghwan Lee (Baylor University) ADMINI 2018.06.05 601
263 Regular 5/29 (Tue) Prof. Sang Ho Oh (Yonsei Univ. College of Medicine) ADMINI 2018.05.29 583
262 Regular 5/15 (Tue) Prof. Myung Gil Kim (Dept. of Chemistry, Chung-Ang Univ.) ADMINI 2018.05.15 792
261 Regular 5/8 (Tue) Prof. Ju-Young Kim (UNIST MSE) ADMINI 2018.05.09 706
260 Regular 5/1 (Tue) Prof. Duk-Yong Yoon (KAIST MSE) ADMINI 2018.04.30 1165
259 Regular 4/24 (Tue) Kyeong Ho Chang (CEO, Innox Advanced Materials) ADMINI 2018.04.23 734
258 Regular 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 807
257 Regular 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 690
256 Regular 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 643
255 Regular 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 543
254 Regular 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 509
253 Regular 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 431
252 Regular 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 494
» Special 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 452
250 Special 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) file ADMINI 2018.02.05 458
249 Special 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 523
248 Special 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 525
247 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 541
246 Regular 11/28 (Tue) Dr. Woo-Jin Lee (Ministry of Science and ICT.) ADMINI 2017.11.24 1184
245 Special 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 850
Board Pagination ‹ Prev 1 2 3 4 5 6 7 8 9 10 ... 15 Next ›
/ 15

Designed by sketchbooks.co.kr / sketchbook5 board skin

나눔글꼴 설치 안내


이 PC에는 나눔글꼴이 설치되어 있지 않습니다.

이 사이트를 나눔글꼴로 보기 위해서는
나눔글꼴을 설치해야 합니다.

설치 Cancel

Sketchbook5, 스케치북5

Sketchbook5, 스케치북5

Sketchbook5, 스케치북5

Sketchbook5, 스케치북5