신소재공학과

Seminar

Date 20171114 

■ Title:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ Speaker: Prof. Jae-Byum Chang (Dept. of Biomedical Engineering, Sungkyunkwan Univ)  

 

■ Date and time: 14th of November, (Tue) 16:00

 

■ Venue: Applied Engineering Dpt. Bd W1 Multimedia Lecture Hall (1st Floor)

 

 Host : Prof. Yeon Sik Jung

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.


No. Subject Author Date Views
Notice 7/13 (Fri) Prof. Dong Ryeol Whang (Johannes Kepler University Linz, Austria) ADMINI 2018.07.09 64
Notice 6/5 (Tue) Prof. Sunghwan Lee (Baylor University) ADMINI 2018.06.05 271
245 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 815
244 11/21 (Tue) Dr. Young Ki Lee (ETRI) file ADMINI 2017.11.20 1006
243 11/22 (Wed) Prof. Sung Hoon Kang (Johns Hopkins University) file ADMINI 2017.11.20 1238
242 11/21 (Tue) Dr. Byoung Koun Min, (KIST) ADMINI 2017.11.17 1369
241 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 728
240 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 710
» 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 723
238 11/7 (Tue) Prof. Su-Mi Hur (Chonnam National University) ADMINI 2017.11.05 548
237 10/31 (Tue) Director Jang Sae Young (Samsung Electronics) ADMINI 2017.10.26 554
236 11/6 (Mon) Prof. Redouane Borsali (Grenoble Alpes University) ADMINI 2017.10.26 654
235 11/6 ~ 7 Prof. Erik Luijten (Northwestern University) file ADMINI 2017.10.25 610
234 10/23 ~ 24 Prof. Paul C. McIntyre (Stanford University) file ADMINI 2017.10.25 536
233 10/24 (Tue) Prof. Sun-Jae Kim (Sejong, Univ) ADMINI 2017.10.20 968
232 10/19 (Thu) Prof. Sang Cheol, Jung (Suncheon University) ADMINI 2017.10.18 1310
231 10/10 (Tue) Dr. Joo Choi (POSCO) ADMINI 2017.09.29 2214
230 9/26 (Tue) Prof. Tae-Woo Lee (Seoul National University) ADMINI 2017.09.22 1124
229 9/19 (Tue) Representative Sang Min Lee (Congressman) ADMINI 2017.09.15 1207
228 9/12 (Tue) Monika Fleischer(Eberhard Karls University Tübingen) ADMINI 2017.09.14 813
227 2017 The Fall Semester Seminar file ADMINI 2017.09.08 2951
226 9/12 (Tue) Prof. Pil Jin, Yoo (Sungkyunkwan Univ) ADMINI 2017.09.08 1340