신소재공학과

행사일 20180220 

■ 제   : Hydrogel-based tissue imaging:

              How materials science is revolutionizing neuroscience, biology, and medicine

 

■ 연   : Prof. Jae-Byum Chang (Sungkyunkwan Univ.) 


■ 일   : 2018년 2월 20일(화) 오후 4시 

 

■ 장   : 응용공학동 (W1-1) 2427호 

 

■ Abstract :

Our understanding on human organs is very limited. Human organs are made of millions of cells and the molecular profiles of cells, such as numbers and sub-cellular locations of various biomolecules (proteins, mRNAs, DNAs, etc.) are precisely controlled over an entire organ. As a result, extracting the complete profiles of biological molecules within an entire organ is essential to understanding the molecular mechanisms of organ function. To achieve such goal, new optical imaging technique, which can image multiple molecular species with a single-molecule resolution over a large volume, is needed.

We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease. In addition, as iExM can be applied to various organs, such as heart, liver, pancreas, lung, intestine, they could be a very useful tool to study and diagnose various diseases, such as cancers and cardiovascular diseases.

No. Subject Author Date Views
Notice 6/5 (Tue) 이성환 교수 (Baylor University) ADMINI 2018.06.05 33
Notice 5/29 (Tue) 오상호 교수 (연세대학교 의과대학 피부과학교실) ADMINI 2018.05.29 28
Notice 5/15 (Tue) 김명길 교수 (중앙대 화학과) ADMINI 2018.05.15 74
Notice 5/8 (Tue) 김주영 교수 (UNIST 신소재공학부) ADMINI 2018.05.09 80
Notice 5/1 (Tue) 윤덕용 명예교수 (KAIST 신소재공학과) ADMINI 2018.04.30 74
Notice 4/24 (Tue) 장경호 대표이사 (이녹스첨단소재, Innox Advanced Materials) ADMINI 2018.04.23 83
Notice 4/10 (Tue) 유기준 교수 (연세대학교 전기전자공학부) ADMINI 2018.04.09 136
Notice 4/3 (Tue) 최병기 마스터 (삼성전자종합기술원) ADMINI 2018.04.02 135
Notice 3/27 (Tue) 홍승범 교수 (KAIST MSE) ADMINI 2018.03.26 153
Notice 3/20 (Tue) 정인호 교수 (서울대학교 재료공학부) ADMINI 2018.03.20 164
Notice 3/13 (Tue) 백종범 교수 (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 151
Notice 3/6 (Tue) 류석영 교수 (KAIST 전산학부) ADMINI 2018.03.05 157
Notice 2/27 (Tue) 한우진 박사 (NASA) ADMINI 2018.02.27 196
» 2/20 (Tue) 장재범 박사 (성균관대학) ADMINI 2018.02.19 188
Notice 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) ADMINI 2018.02.05 186
Notice 2/13 (Tue) 김성규 교수 (Northwestern Univ) ADMINI 2018.01.31 203
255 7/13 (Fri) Prof. Dong Ryeol Whang (Johannes Kepler University Linz,Austria) file ADMINI 2018.07.09 4
254 1/9 (Tue) 변춘원 선임연구원 (ETRI) ADMINI 2018.01.09 192
253 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 192
252 11/28 (Tue) 이우진 서기관 (과학기술정보통신부) ADMINI 2017.11.24 567