신소재공학과

행사일 20171114 

■ 제 목:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ 연 사: 장재범 교수 (성균관대학교 바이오메디컬공학과)  

 

■ 일 시: 11월 14일 (화) 16:00

 

■ 장 소: 응용공학동 (W1) 1층 영상강의실

 

 Host : 정연식 교수

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.

No. Subject Author Date Views
Notice 7/25 (Wed) 김창섭 박사 (Department of MSE, MIT) ADMINI 2018.07.24 16
Notice 7/13 (Fri) Prof. Dong Ryeol Whang (Johannes Kepler University Linz,Austria) ADMINI 2018.07.09 13
Notice 6/5 (Tue) 이성환 교수 (Baylor University) ADMINI 2018.06.05 54
Notice 5/29 (Tue) 오상호 교수 (연세대학교 의과대학 피부과학교실) ADMINI 2018.05.29 51
Notice 5/15 (Tue) 김명길 교수 (중앙대 화학과) ADMINI 2018.05.15 93
252 11/28 (Tue) 이우진 서기관 (과학기술정보통신부) ADMINI 2017.11.24 575
251 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 359
250 11/21 (Tue) 이영기 박사 (한국전자통신연구원) file ADMINI 2017.11.20 525
249 11/22 (Wed) 강성훈 교수 (Johns Hopkins Unviersity) file ADMINI 2017.11.20 574
248 11/21 (Tue) 민병권 센터장 (한국과학기술연구원) ADMINI 2017.11.17 715
247 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 188
246 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 292
» 11/14 (Tue) 장재범 교수 (성균관대학교) ADMINI 2017.11.10 326
244 11/7 (Tue) 허수미 교수 (전남대학교 ADMINI 2017.11.05 215
243 10/31 (Tue) 장세영 상무 (삼성전자) ADMINI 2017.10.26 206
242 11/6 (Mon) Redouane Borsali 교수 (Grenoble Alpes University) ADMINI 2017.10.26 196
241 11/6 ~ 7 Erik Luijten 교수 (Northwestern University) file ADMINI 2017.10.25 229
240 10/23 ~ 24 Paul C. McIntyre 교수 (Stanford University) file ADMINI 2017.10.25 174
239 10/24 (Tue) 김선재 교수 (세종대학교) ADMINI 2017.10.20 547
238 10/19 (Thu) 정상철 교수 (순천대학교) ADMINI 2017.10.18 956
237 10/10 (Tue) 최 주 원장 (POSCO) ADMINI 2017.09.29 1265
236 9/26 (Tue) 이태우 교수 (서울대학교) ADMINI 2017.09.22 541
235 9/19 (Tue) 이상민 의원 (국회의원) ADMINI 2017.09.15 664
234 9/12 (Tue) Monika Fleischer(Eberhard Karls University Tübingen) ADMINI 2017.09.14 337
233 2017 신소재공학과 가을학기 정기세미나 file ADMINI 2017.09.08 1893