신소재공학과

Seminar

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Date 20180220

■ Title : Hydrogel-based tissue imaging:

            How materials science is revolutionizing neuroscience, biology, and medicine

 

■ Speaker : Prof. Jae-Byum Chang (Sungkyunkwan Univ.) 


■ Date and time : 20th of February, 2018 (Tue) 16:00 

 

■ Venue : Applied Engineering Department (W1-1) Room 2427 

 

■ Abstract :

Our understanding on human organs is very limited. Human organs are made of millions of cells and the molecular profiles of cells, such as numbers and sub-cellular locations of various biomolecules (proteins, mRNAs, DNAs, etc.) are precisely controlled over an entire organ. As a result, extracting the complete profiles of biological molecules within an entire organ is essential to understanding the molecular mechanisms of organ function. To achieve such goal, new optical imaging technique, which can image multiple molecular species with a single-molecule resolution over a large volume, is needed.

We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease. In addition, as iExM can be applied to various organs, such as heart, liver, pancreas, lung, intestine, they could be a very useful tool to study and diagnose various diseases, such as cancers and cardiovascular diseases.


List of Articles
No. Category Subject Author Date Views
Notice Regular 6/4 (Tue) Prof. SangBum Kim (Dept. of MSE, SNU) ADMINI 2019.05.31 477
Notice Regular 5/28 (Tue) Prof. Seok Su Sohn (Dept. of MSE, Korea Univ.) ADMINI 2019.05.24 446
Notice Regular 5/21 (Tue) Emeritus Prof. Joong Keun Park (Dept. of MSE, KAIST) ADMINI 2019.05.17 454
Notice Regular 5/17 (Tue) Prof. Su-Hyun Gong (Dept. of Physics, Korea Univ.) ADMINI 2019.05.10 950
Notice Special 5/7 (Tue) Prof. Dr. -ing. Dierk Raabe (MPIE) ADMINI 2019.04.30 763
Notice Regular 4/30 (Tue) Prof. Min Hyuk Park (School of MSE, Pusan National Univ.) ADMINI 2019.04.25 547
Notice Regular 4/23 (Tue) Prof. Seungwu Han (Dept. of MSE, SNU) ADMINI 2019.04.19 308
258 Regular 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 870
257 Regular 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 721
256 Regular 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 665
255 Regular 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 597
254 Regular 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 555
253 Regular 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 449
252 Regular 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 530
» Special 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 502
250 Special 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) file ADMINI 2018.02.05 548
249 Special 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 568
248 Special 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 555
247 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland file ADMINI 2017.12.22 574
246 Regular 11/28 (Tue) Dr. Woo-Jin Lee (Ministry of Science and ICT.) ADMINI 2017.11.24 1219
245 Special 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 922
244 Special 11/21 (Tue) Dr. Young Ki Lee (ETRI) file ADMINI 2017.11.20 1065
243 Special 11/22 (Wed) Prof. Sung Hoon Kang (Johns Hopkins University) file ADMINI 2017.11.20 1359
242 Regular 11/21 (Tue) Dr. Byoung Koun Min, (KIST) ADMINI 2017.11.17 1496
241 Special 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 1858
240 Special 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 803
239 Regular 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 765
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