신소재공학과

Seminar

Date 20171114 

■ Title:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ Speaker: Prof. Jae-Byum Chang (Dept. of Biomedical Engineering, Sungkyunkwan Univ)  

 

■ Date and time: 14th of November, (Tue) 16:00

 

■ Venue: Applied Engineering Dpt. Bd W1 Multimedia Lecture Hall (1st Floor)

 

 Host : Prof. Yeon Sik Jung

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.


No. Subject Author Date Views
Notice 4/24 (Tue) Kyeong Ho Chang (CEO, Innox Advanced Materials) ADMINI 2018.04.23 3
Notice 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 96
Notice 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 104
Notice 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 150
Notice 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 154
Notice 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 163
Notice 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 138
Notice 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 195
Notice 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 155
Notice 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) ADMINI 2018.02.05 163
Notice 2/13 (Tue) Prof. Sungkyu Kim (Northwestern Univ) ADMINI 2018.01.31 222
Notice 1/9 (Tue) Dr. Choon Won Byeon, ETRI ADMINI 2018.01.09 276
Notice 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland ADMINI 2017.12.22 300
Notice 2017 The Fall Semester Seminar ADMINI 2017.09.08 2803
» 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 711
237 11/7 (Tue) Prof. Su-Mi Hur (Chonnam National University) ADMINI 2017.11.05 524
236 10/31 (Tue) Director Jang Sae Young (Samsung Electronics) ADMINI 2017.10.26 544
235 11/6 (Mon) Prof. Redouane Borsali (Grenoble Alpes University) ADMINI 2017.10.26 636
234 11/6 ~ 7 Prof. Erik Luijten (Northwestern University) file ADMINI 2017.10.25 593
233 10/23 ~ 24 Prof. Paul C. McIntyre (Stanford University) file ADMINI 2017.10.25 520
232 10/24 (Tue) Prof. Sun-Jae Kim (Sejong, Univ) ADMINI 2017.10.20 951
231 10/19 (Thu) Prof. Sang Cheol, Jung (Suncheon University) ADMINI 2017.10.18 1297
230 10/10 (Tue) Dr. Joo Choi (POSCO) ADMINI 2017.09.29 2196
229 9/26 (Tue) Prof. Tae-Woo Lee (Seoul National University) ADMINI 2017.09.22 1104
228 9/19 (Tue) Representative Sang Min Lee (Congressman) ADMINI 2017.09.15 1196
227 9/12 (Tue) Monika Fleischer(Eberhard Karls University Tübingen) ADMINI 2017.09.14 799
226 9/12 (Tue) Prof. Pil Jin, Yoo (Sungkyunkwan Univ) ADMINI 2017.09.08 1329
225 9/11 (Mon) Won Joon, Cho (Lawrence Berkeley National Lab, Berkeley) ADMINI 2017.09.06 1335
224 9/13 (Wed) Dr. In Kyung Yoo (SAIT) ADMINI 2017.09.06 1561
223 8/24 (Tue) Prof. Jong Min Yuk (MSE KAIST) ADMINI 2017.08.24 1581
222 6/16 (Fri) Dr. Bok Yeop Ahn (Harvard University) ADMINI 2017.06.13 3132
221 6/12 (Mon) Prof. C. Suryanarayana (University of Central Florida) ADMINI 2017.06.05 1307
220 6/2 (fri) Robert M. Ulfig (CAMECA Instruments Inc) ADMINI 2017.06.01 1263