신소재공학과

행사일 20171114 

■ 제 목:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ 연 사: 장재범 교수 (성균관대학교 바이오메디컬공학과)  

 

■ 일 시: 11월 14일 (화) 16:00

 

■ 장 소: 응용공학동 (W1) 1층 영상강의실

 

 Host : 정연식 교수

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.

No. Subject Author Date Views
Notice 5/15 (Tue) 김명길 교수 (중앙대 화학과) ADMINI 2018.05.15 22
Notice 5/8 (Tue) 김주영 교수 (UNIST 신소재공학부) ADMINI 2018.05.09 23
Notice 5/1 (Tue) 윤덕용 명예교수 (KAIST 신소재공학과) ADMINI 2018.04.30 28
Notice 4/24 (Tue) 장경호 대표이사 (이녹스첨단소재, Innox Advanced Materials) ADMINI 2018.04.23 43
Notice 4/10 (Tue) 유기준 교수 (연세대학교 전기전자공학부) ADMINI 2018.04.09 93
Notice 4/3 (Tue) 최병기 마스터 (삼성전자종합기술원) ADMINI 2018.04.02 94
Notice 3/27 (Tue) 홍승범 교수 (KAIST MSE) ADMINI 2018.03.26 115
Notice 3/20 (Tue) 정인호 교수 (서울대학교 재료공학부) ADMINI 2018.03.20 122
Notice 3/13 (Tue) 백종범 교수 (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 110
Notice 3/6 (Tue) 류석영 교수 (KAIST 전산학부) ADMINI 2018.03.05 118
Notice 2/27 (Tue) 한우진 박사 (NASA) ADMINI 2018.02.27 152
Notice 2/20 (Tue) 장재범 박사 (성균관대학) ADMINI 2018.02.19 148
Notice 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) ADMINI 2018.02.05 139
Notice 2/13 (Tue) 김성규 교수 (Northwestern Univ) ADMINI 2018.01.31 162
Notice 1/9 (Tue) 변춘원 선임연구원 (ETRI) ADMINI 2018.01.09 164
Notice 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland ADMINI 2017.12.22 164
Notice 2017 신소재공학과 가을학기 정기세미나 ADMINI 2017.09.08 1868
247 11/21 (Tue) 민병권 센터장 (한국과학기술연구원) ADMINI 2017.11.17 708
246 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 182
245 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 286
» 11/14 (Tue) 장재범 교수 (성균관대학교) ADMINI 2017.11.10 319
243 11/7 (Tue) 허수미 교수 (전남대학교 ADMINI 2017.11.05 204
242 10/31 (Tue) 장세영 상무 (삼성전자) ADMINI 2017.10.26 197
241 11/6 (Mon) Redouane Borsali 교수 (Grenoble Alpes University) ADMINI 2017.10.26 190
240 11/6 ~ 7 Erik Luijten 교수 (Northwestern University) file ADMINI 2017.10.25 222
239 10/23 ~ 24 Paul C. McIntyre 교수 (Stanford University) file ADMINI 2017.10.25 168
238 10/24 (Tue) 김선재 교수 (세종대학교) ADMINI 2017.10.20 539
237 10/19 (Thu) 정상철 교수 (순천대학교) ADMINI 2017.10.18 950
236 10/10 (Tue) 최 주 원장 (POSCO) ADMINI 2017.09.29 1259
235 9/26 (Tue) 이태우 교수 (서울대학교) ADMINI 2017.09.22 537
234 9/19 (Tue) 이상민 의원 (국회의원) ADMINI 2017.09.15 660
233 9/12 (Tue) Monika Fleischer(Eberhard Karls University Tübingen) ADMINI 2017.09.14 331
232 9/12 (Tue) 유필진 교수 (성균관대학교) ADMINI 2017.09.08 743
231 9/11 (Mon) 조원준 박사 (Lawrence Berkeley National Lab, Berkeley) ADMINI 2017.09.06 672
230 9/13 (Wed) 유인경 박사 (SAIT) ADMINI 2017.09.06 770
229 8/24 (Tue) 육종민 교수 (KAIST 신소재공학과) ADMINI 2017.08.24 960