신소재공학과

행사일 20171114 

■ 제 목:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ 연 사: 장재범 교수 (성균관대학교 바이오메디컬공학과)  

 

■ 일 시: 11월 14일 (화) 16:00

 

■ 장 소: 응용공학동 (W1) 1층 영상강의실

 

 Host : 정연식 교수

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.

No. Subject Author Date Views
Notice 2/20 (Tue) 장재범 박사 (성균관대학) ADMINI 2018.02.19 9
Notice 2/8 (Thu) Prof. Pei-Chen Su (Nanyang Technological University) ADMINI 2018.02.05 5
Notice 2/13 (Tue) 김성규 교수 (Northwestern Univ) ADMINI 2018.01.31 34
Notice 1/9 (Tue) 변춘원 선임연구원 (ETRI) ADMINI 2018.01.09 34
Notice 12/23 (Sat) Prof. Liangbing (Bing) Hu/Univ. of Maryland ADMINI 2017.12.22 43
Notice 2017 신소재공학과 가을학기 정기세미나 ADMINI 2017.09.08 1725
252 11/28 (Tue) 이우진 서기관 (과학기술정보통신부) ADMINI 2017.11.24 494
251 11/24 (Fri) Takashi NOGUCH (University of the Ryukyus) ADMINI 2017.11.23 298
250 11/21 (Tue) 이영기 박사 (한국전자통신연구원) file ADMINI 2017.11.20 463
249 11/22 (Wed) 강성훈 교수 (Johns Hopkins Unviersity) file ADMINI 2017.11.20 513
248 11/21 (Tue) 민병권 센터장 (한국과학기술연구원) ADMINI 2017.11.17 664
247 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 141
246 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 246
» 11/14 (Tue) 장재범 교수 (성균관대학교) ADMINI 2017.11.10 280
244 11/7 (Tue) 허수미 교수 (전남대학교 ADMINI 2017.11.05 162
243 10/31 (Tue) 장세영 상무 (삼성전자) ADMINI 2017.10.26 158
242 11/6 (Mon) Redouane Borsali 교수 (Grenoble Alpes University) ADMINI 2017.10.26 169
241 11/6 ~ 7 Erik Luijten 교수 (Northwestern University) file ADMINI 2017.10.25 198
240 10/23 ~ 24 Paul C. McIntyre 교수 (Stanford University) file ADMINI 2017.10.25 149
239 10/24 (Tue) 김선재 교수 (세종대학교) ADMINI 2017.10.20 514
238 10/19 (Thu) 정상철 교수 (순천대학교) ADMINI 2017.10.18 936