신소재공학과

Seminar

Date 20171114 

■ Title:  How materials science changes brain science:

            20-nm resolution optical imaging of brain via swellable hydrogel


■ Speaker: Prof. Jae-Byum Chang (Dept. of Biomedical Engineering, Sungkyunkwan Univ)  

 

■ Date and time: 14th of November, (Tue) 16:00

 

■ Venue: Applied Engineering Dpt. Bd W1 Multimedia Lecture Hall (1st Floor)

 

 Host : Prof. Yeon Sik Jung

  

 Abstract :   

The identification and localization of biomolecules, throughout entire brain circuits, with nanoscale precision would enable many fundamental insights into the mechanisms underlying the operation of normal and pathological neural networks. We recently discovered that we could physically magnify specimens by embedding them in a dense swellable polymer, anchoring key biomolecules to the polymer mesh, and adding water to swell the polymer, a process we call ‘expansion microscopy’1. Despite the high isotropy of the expansion process, the initial polymer recipe enabled just 4-4.5x expansion, or roughly 60-70 nm spatial resolution. Ideally it would be possible to improve the expansion chemistry so as to enable, ultimately, the imaging of membrane boundaries, as well as protein complexes. Here, we report on an iterative ExM (iExM) chemistry that can achieve ~20x physical magnification of mouse brain tissues, or 20-nm lateral resolution on conventional optical microscopes2. As with the first version of ExM, iExM-processed samples are optically clear. Thus, iExM may be useful for imaging nanoscale neuronal structures such as synaptic clefts over entire neural circuits in intact mammalian tissues. Brain circuit mapping using iterative ExM may open up a variety of insights into the underpinnings of behavior, cognition, and disease.

 

Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548.

Chang, J.-B., Chen, F., Yoon, Y.-G., Jung, E. E., Babcock H., Kang J.-S., Asano S., Suk H.-J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. (2017) Iterative expansion microscopy, Nature Methods 14:593-599.


No. Subject Author Date Views
Notice 6/5 (Tue) Prof. Sunghwan Lee (Baylor University) ADMINI 2018.06.05 84
Notice 5/29 (Tue) Prof. Sang Ho Oh (Yonsei Univ. College of Medicine) ADMINI 2018.05.29 90
Notice 5/15 (Tue) Prof. Myung Gil Kim (Dept. of Chemistry, Chung-Ang Univ.) ADMINI 2018.05.15 305
Notice 5/8 (Tue) Prof. Ju-Young Kim (UNIST MSE) ADMINI 2018.05.09 374
Notice 5/1 (Tue) Prof. Duk-Yong Yoon (KAIST MSE) ADMINI 2018.04.30 540
Notice 4/24 (Tue) Kyeong Ho Chang (CEO, Innox Advanced Materials) ADMINI 2018.04.23 514
Notice 4/10 (Tue) Prof. Ki Jun, Yu (Yonsei Univ., School of Electrical and Electronic Engineering) ADMINI 2018.04.09 595
Notice 4/3 (Tue) Master Byung-Gi Choi (Samsung Advanced Institute of Technology) ADMINI 2018.04.02 480
Notice 3/27 (Tue) Prof. Seungbum Hong (KAIST MSE) ADMINI 2018.03.26 450
Notice 3/20 (Tue) Prof. Jung In-Ho (Seoul National University, MSE) ADMINI 2018.03.20 319
Notice 3/13 (Tue) Prof. Jong-Beom Baek (School of Energy and Chemical Engineering, UNIST) ADMINI 2018.03.13 316
Notice 3/6 (Tue) Prof. Seok-Young Ryu (KAIST CS) ADMINI 2018.03.05 281
Notice 2/27 (Tue) Dr. Jin-Woo Han (NASA) ADMINI 2018.02.27 341
Notice 2/20 (Tue) Dr. Jae Beom Jang (Sungkyunkwan Univ) ADMINI 2018.02.19 309
244 11/21 (Tue) Dr. Young Ki Lee (ETRI) file ADMINI 2017.11.20 1002
243 11/22 (Wed) Prof. Sung Hoon Kang (Johns Hopkins University) file ADMINI 2017.11.20 1233
242 11/21 (Tue) Dr. Byoung Koun Min, (KIST) ADMINI 2017.11.17 1361
241 11/13 (Mon) Dr. Hans Aage Hiuler (Danish Power System) ADMINI 2017.11.10 724
240 11/13 (Mon) Prof. Jens Oluf Jensen (Technical University of Denmark) ADMINI 2017.11.10 694
» 11/14 (Tue) Prof. Jae-Byum Chang (Sungkyunkwan Univ) ADMINI 2017.11.10 718
238 11/7 (Tue) Prof. Su-Mi Hur (Chonnam National University) ADMINI 2017.11.05 538
237 10/31 (Tue) Director Jang Sae Young (Samsung Electronics) ADMINI 2017.10.26 550
236 11/6 (Mon) Prof. Redouane Borsali (Grenoble Alpes University) ADMINI 2017.10.26 650
235 11/6 ~ 7 Prof. Erik Luijten (Northwestern University) file ADMINI 2017.10.25 606
234 10/23 ~ 24 Prof. Paul C. McIntyre (Stanford University) file ADMINI 2017.10.25 531
233 10/24 (Tue) Prof. Sun-Jae Kim (Sejong, Univ) ADMINI 2017.10.20 962
232 10/19 (Thu) Prof. Sang Cheol, Jung (Suncheon University) ADMINI 2017.10.18 1306
231 10/10 (Tue) Dr. Joo Choi (POSCO) ADMINI 2017.09.29 2209
230 9/26 (Tue) Prof. Tae-Woo Lee (Seoul National University) ADMINI 2017.09.22 1120
229 9/19 (Tue) Representative Sang Min Lee (Congressman) ADMINI 2017.09.15 1205
228 9/12 (Tue) Monika Fleischer(Eberhard Karls University Tübingen) ADMINI 2017.09.14 809
227 2017 The Fall Semester Seminar file ADMINI 2017.09.08 2946
226 9/12 (Tue) Prof. Pil Jin, Yoo (Sungkyunkwan Univ) ADMINI 2017.09.08 1338
225 9/11 (Mon) Won Joon, Cho (Lawrence Berkeley National Lab, Berkeley) ADMINI 2017.09.06 1345